Th1 and Th2 immune response to P30 and ROP18 peptides in human toxoplasmosis Academic Article

abstract

  • We determined the specific lymphocyte proliferative response and cytokine profile production regarding Toxoplasma P30 (2017 from virulent and non-virulent strain) and ROP18 protein-derived peptides (from clonal lineages I, II and III) in 19 patients having ocular toxoplasmosis, five suffering chronic asymptomatic infection, nine with congenital toxoplasmosis and eight Toxoplasma negative people. A Beckman Coulter FC500 flow cytometer was used for determining antigen-specific T cells (CD3+ CD4+ or CD3+ CD8+ cells) in peripheral blood culture. IFN γ and IL10 levels were determined in culture supernatants. Specific CD4+ and CD8+ T cell response to total antigen and P30- and ROP18-derived peptides was observed in infected people. Ocular toxoplasmosis patients had a preferential Th2 response after antigenic stimulation. Non-virulent peptide 2017 was able to shift response toward Th1 in congenitally infected children and virulent peptide 2017 induced a Th2 response in chronically infected, asymptomatic people. An immune response in human toxoplasmosis after ex vivo antigenic stimulation was Th1- or Th2-skewed, depending on a patient’s clinical condition. Colombian ocular toxoplasmosis patients’ immune response was Th2-skewed, regardless of the nature of antigen stimulus.

publication date

  • 2014/1/1

keywords

  • Antigens
  • Asymptomatic Infections
  • Blood Culture
  • Congenital Toxoplasmosis
  • Cytokines
  • Interleukin-10
  • Lymphocytes
  • Ocular Toxoplasmosis
  • Peptides
  • Proteins
  • T-Lymphocytes
  • Toxoplasma
  • Toxoplasmosis

International Standard Serial Number (ISSN)

  • 0300-8584

number of pages

  • 8

start page

  • 315

end page

  • 322