High level of conservation in Plasmodium vivax merozoite surface protein 4 (PvMSP4) Academic Article

journal

  • Infection, Genetics and Evolution

abstract

  • Plasmodium vivax merozoite surface protein 4 (PvMSP4) has been considered to be a promising malarial vaccine candidate. The antigenic diversity displayed by parasite populations is one of the main factors limiting the efficacy of asexual-stage anti-malarial vaccine candidates. The present study is the first characterising PvMSP4 polymorphism. P. vivax isolates were collected from endemic areas in Colombia and diversity and selection pattern studies were carried out. Overall conservation in this protein was remarkable. Changes were only found in exons I and II, the former only having single nucleotide polymorphisms (SNPs) whilst the latter showed variations in tandem repeat number caused by exon II slippage. The remaining regions (EGF-like domain, GPI anchor and intron) were completely conserved. Selection and neutrality tests carried out over variant exons indicated negative selective forces acting on them. No evidence of intragenic recombination was found. The strong conservation displayed in this molecule by isolates from geographically different regions (Colombia, Salvador and Thailand) stresses its potential importance as a candidate for a vaccine against P. vivax malaria.
  • Plasmodium vivaxmerozoite surface protein 4 (PvMSP4) has been considered to be a promising malarial vaccine candidate. The antigenicdiversity displayed by parasite populations is one of the main factors limiting the efficacy of asexual-stage anti-malarial vaccine candidates.The present study is the first characterising PvMSP4 polymorphism.P. vivaxisolates were collected from endemic areas in Colombia anddiversity and selection pattern studies were carried out. Overall conservation in this protein was remarkable. Changes were only found inexons I and II, the former only having single nucleotide polymorphisms (SNPs) whilst the latter showed variations in tandem repeat numbercaused by exon II slippage. The remaining regions (EGF-like domain, GPI anchor and intron) were completely conserved. Selection andneutrality tests carried out over variant exons indicated negative selective forces acting on them. No evidence of intragenic recombination wasfound. The strong conservation displayed in this molecule by isolates from geographically different regions (Colombia, Salvador andThailand) stresses its potential importance as a candidate for a vaccine againstP. vivaxmalaria.#2004 Elsevier B.V. All rights reserved.

publication date

  • 2005-1-18

edition

  • 5

keywords

  • Antigenic Variation
  • Antimalarials
  • Colombia
  • Epidermal Growth Factor
  • Exons
  • Genetic Recombination
  • Introns
  • Malaria Vaccines
  • Parasites
  • Plasmodium merozoite surface protein 4
  • Plasmodium vivax
  • Population
  • Proteins
  • Single Nucleotide Polymorphism
  • Tandem Repeat Sequences
  • Thailand
  • Vaccines
  • Vivax Malaria
  • anchor
  • antigenic variation
  • antimalarials
  • exons
  • genetic polymorphism
  • intragenic recombination
  • introns
  • limiting factor
  • malaria
  • merozoites
  • parasite
  • parasites
  • polymorphism
  • protein
  • proteins
  • recombination
  • single nucleotide polymorphism
  • surface proteins
  • tandem repeat sequences
  • test
  • testing
  • vaccine
  • vaccines

International Standard Serial Number (ISSN)

  • 1567-1348

number of pages

  • 8

start page

  • 354

end page

  • 361