Immunogenicity and protection-inducing ability of recombinant Plasmodium vivax rhoptry-associated protein 2 in Aotus monkeys: A potential vaccine candidate Academic Article

journal

  • Vaccine

abstract

  • Rhoptry proteins have been extensively shown to be important in invasion and parasitophorous vacuole (PV) formation. This work evaluates the immunogenicity and protective efficacy of Plasmodium vivax RAP2 in the non-human Aotus primate model, when expressed as a recombinant molecule in E. coli and formulated in Freund and Alum hydroxide adjuvants. Our results show that rPvRAP2 is immunogenic in both formulations, finding a trend of higher cytokine levels in immunized monkeys, specially in IL-4 levels (using Freund's adjuvant) and IL-5 (using Alum hydroxide). RAP2 is suggested as a P. vivax-vaccine candidate since immunized monkeys exhibited lower parasitemias than control groups after being experimentally challenged with the P. vivax VCG-I strain. © 2009 Elsevier Ltd. All rights reserved.

publication date

  • 2009/5/11

keywords

  • Aotus (Cebidae)
  • Control Groups
  • Cytokines
  • Escherichia coli
  • Freund's Adjuvant
  • Haplorhini
  • Interleukin-4
  • Interleukin-5
  • Parasitemia
  • Plasmodium rhoptry associated protein
  • Plasmodium vivax
  • Primates
  • Proteins
  • Vaccines
  • Vacuoles
  • adjuvants
  • alum
  • aluminum sulfate
  • animal models
  • cytokines
  • hydroxide ion
  • hydroxides
  • immune response
  • interleukin-4
  • interleukin-5
  • monkeys
  • parasitemia
  • proteins
  • rhoptry
  • rhoptry proteins
  • vaccines
  • vacuoles

International Standard Serial Number (ISSN)

  • 0264-410X

number of pages

  • 7

start page

  • 2870

end page

  • 2876