Cultivo primario de queratinocitos humanos sembrados en submucosa intestinal porcina Academic Article


  • Revista ciencias de la salud


  • Tissue engineering, in the fields of skin regeneration, seeks to overcome the limitations associated with the use of immediate auto-grafts, since choosing the donor region of the patient constitutes a risk for the patient himself and is insufficient if the injury that has to be repaired is very large. The use of the pig's small intestine submucosa (SIS) has being proved as a filling biomaterial to treat injuries, because of its special composition, it lowers pain and inflammation since its first application and it favours early mobility to the wounded area. The present study developed a protocol for the primary culture of human keratinocytes from infant foreskins, and used small intestinal submucosa (SIS) like culture substrate. Cellular adherence potential and proliferation capability of the keratinocytes over this substrate was evaluated.

publication date

  • 2008-1-1


  • 6


  • Biocompatible Materials
  • Foreskin
  • Inflammation
  • Intestines
  • Keratinocytes
  • Pain
  • Regeneration
  • Skin
  • Small Intestine
  • Swine
  • Tissue Donors
  • Tissue Engineering
  • Transplants
  • Wounds and Injuries
  • engineering
  • infant
  • pain
  • proliferation

International Standard Serial Number (ISSN)

  • 1692-7273

number of pages

  • 15

start page

  • 8

end page

  • 22