Identifying gp85- regions involved in Epstein- Barr virus binding to Blymphocytes. Academic Article

journal

  • Biochemical and Biophysical Research Communications

abstract

  • Epstein–Barr virus lacking glycoprotein gp85 cannot infect B-cells and epithelial cells. The gp85 belongs to the molecular complex required for virus invasion of B-lymphocyte or epithelial cells. Moreover, there is evidence that gp85 is necessary for virus attachment to epithelial cells. Thirty-six peptides from the entire gp85-sequence were tested in epithelial and lymphoblastoid cell line binding assays to identify gp85-regions involved in virus–cell interaction. Five of these peptides presented high binding activity to Raji, Ramos, P3HR-1, and HeLa cells, but not to erythrocytes; Raji-cell affinity constants were between 80 and 140 nM. Of these five peptides, 11435 (181TYKRVTEKGDEHVLSLVFGK200), 11436 (201TKDLPDLRGPFSYPSLTSAQ220), and 11438 (241YFVPNLKDMFSRAVTMTAAS260) bound to a 65 kDa protein on Raji-cell surface. These peptides and antibodies induced by them (recognising live EBV-infected cells) inhibited Epstein–Barr virus interaction with cord blood lymphocytes. It is thus probable that gp85-regions defined by peptides 11435, 11436, and 11438 are involved in EBV invasion of B-lymphocytes.

publication date

  • 2004-1-1

edition

  • 319

keywords

  • Antibodies
  • Assays
  • B-Lymphocytes
  • Blood
  • Cell Line
  • Cells
  • Epithelial Cells
  • Erythrocytes
  • Fetal Blood
  • Glycoproteins
  • HeLa Cells
  • Human Herpesvirus 4
  • Lymphocytes
  • Peptides
  • Proteins
  • Virus Attachment
  • Viruses

International Standard Serial Number (ISSN)

  • 0006-291X

number of pages

  • 8

start page

  • 221

end page

  • 229