In vitro and in vivo studies for assessing the immune response and protection-inducing ability conferred by Fasciola hepatica-derived synthetic peptides containing B- and T-cell epitopes Academic Article

abstract

  • Fasciolosis is considered the most widespread trematode disease affecting grazing animals around the world; it is currently recognised by the World Health Organisation as an emergent human pathogen. Triclabendazole is still the most effective drug against this disease; however, resistant strains have appeared and developing an effective vaccine against this disease has increasingly become a priority. Several bioinformatics tools were here used for predicting B- and T-cell epitopes according to the available data for Fasciola hepatica protein amino acid sequences. BALB/c mice were immunised with the synthetic peptides by using the ADAD vaccination system and several immune response parameters were measured (antibody titres, cytokine levels, T-cell populations) to evaluate their ability to elicit an immune response. Based on the immunogenicity results so obtained, seven peptides were selected to assess their protection-inducing ability against experimental infection with F. hepatica metacercariae. Twenty-four B- or T-epitope-containing peptides were predicted and chemically synthesised. Immunisation of mice with peptides so-called B1, B2, B5, B6, T14, T15 and T16 induced high levels of total IgG, IgG1 and IgG2a (p

publication date

  • 2014/8/14

keywords

  • Amino Acid Sequence
  • Amino Acids
  • Animals
  • Antibodies
  • Aptitude
  • B-Lymphocyte Epitopes
  • B-lymphocytes
  • Bioinformatics
  • Computational Biology
  • Cytokines
  • Epitopes
  • Fasciola hepatica
  • Health
  • Herbivory
  • Immune System
  • Immunization
  • Immunoglobulin G
  • In Vitro Techniques
  • Infection
  • Metacercariae
  • Pathogens
  • Peptide T
  • Peptides
  • Pharmaceutical Preparations
  • Population
  • Proteins
  • T-Lymphocyte Epitopes
  • T-Lymphocytes
  • T-cells
  • T-lymphocytes
  • Trematoda
  • Vaccination
  • Vaccines
  • World Health Organization
  • amino acid sequences
  • animals
  • antibodies
  • bioinformatics
  • cytokines
  • drugs
  • epitopes
  • fascioliasis
  • grazing
  • immune response
  • immunization
  • in vivo studies
  • infection
  • metacercariae
  • mice
  • pathogens
  • peptide B
  • peptides
  • proteins
  • synthetic peptides
  • triclabendazole
  • vaccination
  • vaccines

International Standard Serial Number (ISSN)

  • 1932-6203