The diagnostic performance of classical molecular tests used for detecting human papillomavirus Academic Article

abstract

  • Cervical samples were evaluated for human papillomavirus (HPV) presence using the hybrid capture-2 (HC2) assay and the polymerase chain reaction (PCR) with three different primer sets (GP5+/6+, MY09/11 and pU1M/2R). PCR results were compared to HC2 and results of all assays were compared to cytological and colposcopy findings. Post-test probability was assessed in individual assays and test combinations. HPV-DNA prevalence was 36.5% with HC2 and 55.2% with PCR. MY09/11 detected HPV-DNA in 38% of samples, GP5+/6+ in 19.1% and pU1M/2R in 16.4%. pU1M/2R and HC2 had the highest concordance (75.31%, k= 0.39 in the whole population; 74.1%, k= 0.5 in women with abnormal cytology). pU1M/2R had the best diagnostic performance, including optimal post-test probabilities and cervical abnormality detection (individually or in a panel of tests). Women positive for pU1M/2R may be at higher risk of disease progression; the assay performance when combined with a Pap smear in cervical cancer screening programs should be evaluated. © 2012 Elsevier B.V..

publication date

  • 2012/10/1

keywords

  • Cell Biology
  • Colposcopy
  • DNA
  • Disease Progression
  • Early Detection of Cancer
  • Human papillomavirus 11
  • Papanicolaou Test
  • Polymerase Chain Reaction
  • Population
  • Uterine Cervical Neoplasms

International Standard Serial Number (ISSN)

  • 0166-0934

number of pages

  • 7

start page

  • 32

end page

  • 38