Efecto de la hipoxia-reoxigenación y las radiaciones ionizantes en la captación de glucosa en líneas tumorales de seno y colon cocultivadas con células endoteliales.
Thesis
Glucose uptake and it´s conversion to lactate plays an important role for tumor metabolism. This phenomena does not depend on oxygen present at the tissue (Warburg effect). This glucose uptake is different between tumors and even into the same tumor. This observation may be depending on tumor microenviroment (fluctuations on oxygen availability and the presence of other non tumoral cell types) and other stress factors associated to the treatments for the disease. We evaluate the effect of hypoxia-reoxigenation (HR) and ionizing radiations (IR) on the glucose uptake, in cell cultures of MCF-7 and HT29 in isolated cultures or cocultured with EAhy926. We found that glucose uptake for the cells exposed to HR were different from that described for the hypoxia conditions. Also we observed that this pattern were modified when tumor cells were cocultured with the endothelial cell line. We identified cell populations in relation to glucose uptake (High, medium, low). We evaluate the nuclear translocation of NRF2 and HIF1a proteins, as an evaluation of hypoxia and cellular stress pathways, finding a possible correlation with breast cancer cells, but no with the colon cell line. This suggest that it may be another pathways involved. The different patterns of glucose uptake and metabolism of tumor cells in relation with glucose uptake, when comparing hypoxia and hypoxia-reoxigenation, could lead to a better dossymmetric planning taking account the variations on oxygen concentration into the tumor.
