Human Papillomavirus Detection from Human Immunodeficiency Virus-Infected Colombian Women's Paired Urine and Cervical Samples Academic Article

abstract

  • Infection, coinfection and type-specific human papillomavirus (HPV) distribution was evaluated in human immunodeficiency virus (HIV)-positive women from paired cervical and urine samples. Paired cervical and urine samples (n = 204) were taken from HIV-positive women for identifying HPV-DNA presence by using polymerase chain reaction (PCR) with three generic primer sets (GP5+/6+, MY09/11 and pU1M/2R). HPV-positive samples were typed for six high-risk HPV (HR-HPV) (HPV-16, -18, -31, -33, -45 and -58) and two low-risk (LR-HPV) (HPV-6/11) types. Agreement between paired sample results and diagnostic performance was evaluated. HPV infection prevalence was 70.6% in cervical and 63.2% in urine samples. HPV-16 was the most prevalent HPV type in both types of sample (66.7% in cervical samples and 62.0% in urine) followed by HPV-31(47.2%) in cervical samples and HPV-58 (35.7%) in urine samples. There was 55.4% coinfection (infection by more than one type of HPV) in cervical samples and 40.2% in urine samples. Abnormal Papanicolau smears were observed in 25.3% of the women, presenting significant association with HPV-DNA being identified in urine samples. There was poor agreement of cervical and urine sample results in generic and type-specific detection of HPV. Urine samples provided the best diagnosis when taking cytological findings as reference. In conclusion including urine samples could be a good strategy for ensuring adherence to screening programs aimed at reducing the impact of cervical cancer, since this sample is easy to obtain and showed good diagnostic performance. © 2013 Munoz et al.

publication date

  • 2013/2/13

keywords

  • Coinfection
  • DNA
  • HIV
  • Human immunodeficiency virus
  • Human papillomavirus 11
  • Human papillomavirus 16
  • Human papillomavirus 18
  • Human papillomavirus 31
  • Human papillomavirus 6
  • Infection
  • Papillomaviridae
  • Papillomavirus Infections
  • Polymerase Chain Reaction
  • Polymerase chain reaction
  • Screening
  • Urine
  • Uterine Cervical Neoplasms
  • Viruses
  • infection
  • mixed infection
  • polymerase chain reaction
  • sampling
  • screening
  • urine
  • uterine cervical neoplasms

International Standard Serial Number (ISSN)

  • 1932-6203