Contemporary cryptic sexuality in Trypanosoma cruzi Academic Article


  • Molecular Ecology


  • Clonal propagation is considered to be the predominant mode of reproduction among many parasitic protozoa. However, this assumption may overlook unorthodox, infrequent or cryptic sexuality. Trypanosoma cruzi, which causes Chagas disease, is known to undergo non-Mendelian genetic exchange in the laboratory. In the field, evidence of extant genetic exchange is limited. In this study, we undertook intensive sampling of T. cruzi Discrete Typing Unit I in endemic eastern Colombia. Using Fluorescence-activated cell sorting, we generated 269 biological clones from 67 strains. Each clone was genotyped across 24 microsatellite loci. Subsequently, 100 representative clones were typed using 10 mitochondrial sequence targets (3.76 Kbp total). Clonal diversity among humans, reservoir hosts and vectors suggested complex patterns of superinfection and/or coinfection in oral and vector-borne Chagas disease cases. Clonal diversity between mother and foetus in a congenital case demonstrates that domestic TcI genotypes are infective in utero. Importantly, gross incongruence between nuclear and mitochondrial markers is strong evidence for widespread genetic exchange throughout the data set. Furthermore, a confirmed mosaic maxicircle sequence suggests intermolecular recombination between individuals as a further mechanism of genetic reassortment. Finally, robust dating based on mitochondrial DNA indicates that the emergence of a widespread domestic TcI clade that we now name TcIDOM (formerly TcIa/VEN Dom) occurred 23 000 ± 12 000 years ago and was followed by population expansion, broadly corresponding with the earliest human migration into the Americas. © 2012 Blackwell Publishing Ltd.

publication date

  • 2012/9/1


  • Americas
  • Chagas Disease
  • Chagas disease
  • Clone Cells
  • Coinfection
  • Colombia
  • Datasets
  • Fetus
  • Flow Cytometry
  • Genetic Recombination
  • Genotype
  • Human Migration
  • Microsatellite Repeats
  • Mitochondrial DNA
  • Names
  • Population
  • Protozoa
  • Reproduction
  • Sexuality
  • Superinfection
  • Trypanosoma cruzi
  • clone
  • clones
  • dating
  • disease reservoirs
  • fetus
  • flow cytometry
  • fluorescence
  • genotype
  • human migration
  • laboratory
  • loci
  • marker
  • microsatellite repeats
  • mitochondrial DNA
  • mixed infection
  • mosaic
  • mouth
  • recombination
  • sampling
  • sexuality
  • sorting
  • superinfection

International Standard Serial Number (ISSN)

  • 0962-1083

number of pages

  • 11

start page

  • 4216

end page

  • 4226