Ecology of Trypanosoma cruzi I genotypes across Rhodnius prolixus captured in Attalea butyracea palms Academic Article

abstract

  • © 2017 Elsevier B.V.Trypanosoma cruzi, the agent of Chagas disease exhibits significant genetic diversity. This parasite is divided into six discrete typing units (DTUs) where T. cruzi I (TcI) is the most widespread in the Americas. TcI genotypes have been associated to domestic and sylvatic cycles of transmission (TcIDom and sylvatic TcI). Due to the importance of the enzootic transmission, we determined the frequency of TcI genotypes present in Rhodnius prolixus captured in different regions of the palm A. butyracea to understand the ecology of the disease and the importance of A. butyracea palms as ecotopes of R. prolixus. Forty A. butyracea palms were sampled (base crown, mid-point and crown) capturing 105 individuals identified as R. prolixus by morphological and molecular barcoding. We conducted molecular detection and typing of T. cruzi across 59 individuals. The results showed that all the insects were infected with TcI; 28.57% were sylvatic TcI, 12.38% TcIDom and 15,24% mixed infections (TcIDom/sylvatic TcI). Statistical analysis showed a similar behavior between TcIDom and mixed infections in the mid-point and at the crown of the palm, being more frequent in the crown, while sylvatic TcI does not seem to have a specific association with any of the sampled areas. These findings are consistent with other studies showing high mobility of the insect vector between different ecotopes, increasing the need to develop improvements in the programs of disease control.

publication date

  • 2017/4/1

keywords

  • Americas
  • Attalea
  • Chagas Disease
  • Chagas disease
  • Coinfection
  • Crowns
  • DNA barcoding
  • Ecology
  • Genotype
  • Insect Vectors
  • Insects
  • Molecular Typing
  • Parasites
  • Rhodnius
  • Rhodnius prolixus
  • Trypanosoma cruzi
  • detection
  • disease control
  • disease control programs
  • ecology
  • genetic diversity
  • genetic variation
  • genotype
  • infection
  • insect
  • insect vectors
  • insects
  • mixed infection
  • parasite
  • parasites
  • programme
  • statistical analysis

International Standard Serial Number (ISSN)

  • 1567-1348

number of pages

  • 5

start page

  • 146

end page

  • 150