Two new species of the damselfly genus Archaeopodagrion, A. recurvatumsp. nov. and A. mayisp. nov., are described from the confluence of the Tropical Andes and the Tumbes-Chocó-Magdalena biodiversity hotspots. Adults differ from the other known species in the shape of female posterior lobe of pronotum and male structures of cerci and paraprocts; the larva differs from other Archaeopodagrion species in the caudal lamellae structure and in the mandibular formula. The two new species are diagnosed, a morphological key to all known males and females in the genus is provided, and geographical distributions are updated. Finally, observations on habitat preferences for each newly described species are provided.