Long-term storage and safe retrieval of human papillomavirus DNA using FTA elute cards Academic Article

journal

  • Journal of Virological Methods

abstract

  • Biobanking or collection and storage of specimens for future research purposes have become an essential tool in many fields of biomedical research and aims to provide a better understanding of disease mechanisms as well as the identification of disease-specific biomarkers that can navigate in complex diseases. In this study, we assessed the use of Flinders Technology Associates (FTA) cards as a long-term storage device for cervical specimens with suspected human papillomavirus (HPV) infections. HPV detection and genotyping results in liquid-based transport media were compared to HPV results from FTA cards. The overall agreement for the presence of any HPV infection between liquid-based medium and FTA cards stored for 1 year at ambient temperature was 100%. Reproducibility analysis of HPV detection and genotyping from FTA cards demonstrated that FTA cards are a reliable medium to store and preserve viral nucleic acids. Biobanking of cervical cells on FTA cards may provide a key resource for epidemiological and retrospective HPV studies.

publication date

  • 2016-3-1

edition

  • 229

keywords

  • Biomarkers
  • Biomedical Research
  • DNA
  • Equipment and Supplies
  • Nucleic Acids
  • Papillomavirus Infections
  • Specimen Handling
  • Technology
  • Temperature

International Standard Serial Number (ISSN)

  • 0166-0934

number of pages

  • 6

start page

  • 60

end page

  • 65