Biomarkers of susceptibility and effect in car painters exposed to organic solvents
Grant
Overview
Affiliation
Research
View All
Overview
abstract
Introduction: Motor vehicle painters are routinely exposed to organic solvents, agents capable of producing mutagenic and carcinogenic effects. Objective: To characterise population susceptibility and evaluate genotoxic effects due to exposure to organic solvents. Methodology: A cross-sectional study was conducted comparing a group of car painters exposed to organic solvents with an unexposed group. Polymorphisms of CYP2E1 and the presence of micronuclei in lymphocytes were determined. Results: 122 workers participated in the study, 62 who worked in car paint shops exposed to solvents and 60 who were not exposed. In relation to questionnaire Q 16, 32% of those exposed reported symptoms suggestive of neurotoxicity. When CYP2E1 polymorphism genotypes and the characteristics assessed in the micronucleus test were compared between the two groups, no statistically significant differences were found. However, when the frequencies of micronuclei, micronuclei, micronuclei cells and nucleo-plasmic bridges between the allele groups c1c1 and c2c2/c1c2 of the CYP2E1 polymorphism were compared in the exposure group, statistically significant differences were found. Conclusions: This study reaffirms that workers exposed to organic solvents with CYP2E1 polymorphisms, specifically with genotype c1c1, are more likely to present mutations in somatic cells, a condition associated with a greater susceptibility to diseases such as cancer.
ultados: Participaron en el estudio 122 trabajadores, 62 que laboraban en talleres de pintura de autos expuestos a solventes y 60 no expuestos. En relación al cuestionario Q 16, el 32% de los expuestos refirieron síntomas sugestivos de neurotoxicidad. Al comparar entre los dos grupos los genotipos del polimorfismo de CYP2E1 y las características evaluadas en la prueba de micronúcleos, no se hallaron diferencias estadísticamente significativas. Sin embargo, cuando en el grupo de expuestos fueron comparadas las frecuencias de micronúcleos, de células micronucleadas y de puentes nucleoplásmicos entre los grupos de alelos c1c1 y c2c2/c1c2 del polimorfismo CYP2E1, se encontraron diferencias estadísticamente significativas. Conclusiones: Este estudio reafirma que los trabajadores expuestos a solventes orgánicos con polimorfismos de CYP2E1, específicamente con genotipo c1c1, tienen mayor probabilidad de presentar mutaciones en las células somáticas, condición asociada con una mayor susceptibilidad a enfermedades como el cáncer.
