Caracterización molecular y fenotípica de bacterias gram negativas resistentes a carbapenemicos por serin carbapenemasas tipo KPC en una institución de cuarto nivel de Bogotá Colombia durante el periodo 2018 a 2021
Thesis
Introduction: Resistance to carbapenems by gram-negative bacteria represents a significant loss of last-line drugs for its management. In addition, the current diagnostic methods for the identification of these mechanisms are not widely used in hospital entities. The knowledge of the local epidemiology allows to guide anticipated treatments, reducing associated morbidity and mortality. Objectives: To describe the distribution of the KPC gene in gram-negative bacteria resistant to carbapenems and its relationship with phenotypic identification and differentiation tests. Methods: An observational, descriptive, cross-sectional study was carried out. The study population consisted of gram-negative bacteria, resistant to carbapenems, with identification of the KPC gene, by molecular panel, in adult patients who were hospitalized at the Fundación cardioinfantil during the years 2018 to 2021. Results: 135 sepsis molecular panels were evaluated, of which 41 detected the KPC gene. K. pneumoniae was the germ mainly found globally (60.9%) and in each of the services studied (ICU 68%, hospitalization 45.5% and emergency room 60%). The phenotypic tests, specifically the Hodge and boronic acid tests, had a 93.8% concordance with the molecular test. Conclusions: K. pneumoniae continues to be the main germ resistant to carbapenems due to KPC-type serine carbapenemases.
