The eukaryotic genome, its reads, and the unfinished assembly Academic Article

abstract

  • In recent years, readily affordable short read sequences provided by next-generation sequencing (NGS) have become longer and more accurate. This has led to a jump in interest in the utility of NGS-only approaches for exploring eukaryotic genomes. The concept of a static, 'finished' genome assembly, which still appears to be a faraway goal for many eukaryotes, is yielding to new paradigms. We here motivate an object-view concept where the raw reads are the main, fixed object, and assemblies with their annotations take a role of dynamically changing and modifiable views of that object.

publication date

  • 2013-7-11

edition

  • 587

keywords

  • Eukaryota
  • Genome
  • High-Throughput Nucleotide Sequencing

number of pages

  • 4

start page

  • 2090

end page

  • 2093