Prueba molecular Genotype®MTBDRplus, una alternativa para la detección rápida de tuberculosis multidrogorresistente Academic Article


  • Revista Peruana de Medicina Experimental y Salud Publica


  • The Genotype®MTBDRplus molecular test is a method that allows identifcation of the most frequent mutations associated with resistance to major frst-line antituberculosis drugs, Isoniazid (INH) and Rifampicin (RfP). The aim of this study was to evaluate the performance of the molecular test with culture and smear- positive sputum samples. We evaluated 95 cultures and 100 sputum samples with resistance profles previously determined by the reference method "Agar Plate Proportions" (APP). The molecular test from cultures showed a sensitivity of 100%, 97,5% and 96,97% for RIf, INH and MDR respectively while from sputums the sensitivity was 95,65%, 96,77% and 95,24% for RIf, INH and MDR respectively. We conclude that the molecular test Genotype®MTBDRplus is a very useful tool to detect resistance to isoniazid and rifampicin simultaneously (MDR-TB) in up to 72 hours from sputum samples or cultures.

publication date

  • 2012-3-1


  • 29


  • Agar
  • Isoniazid
  • Multiple Drug Resistance
  • Mutation
  • Pharmaceutical Preparations
  • Rifampin
  • Sputum
  • Tuberculosis

International Standard Serial Number (ISSN)

  • 1726-4634

number of pages

  • 7

start page

  • 92

end page

  • 98