Nanopartículas y radioterapia: estudio del posible efecto radiosensibilizador de puntos de carbono dopados con iones de Gadolinio en células derivadas de Glioblastoma (U87)
Thesis
Glioblastoma is one of the most aggressive tumors and difficult to treat, due to its location, its morphology, and its radioresistant behavior. Being located in the brain, it is not possible to completely remove it during the surgical procedure, which is why, in most cases, it is necessary to use adjuvant treatments such as radiotherapy after surgery. However, by generating resistance to this treatment, the risks of tumor growth and propagation are very high, which generates the need to implement a radiosensitizing agent that allows potentiating the effects of radiotherapy. In this work, the radiosensitizing effect of carbon dots doped with Gd3+ ions (PC-Gd3+) in cells derived from glioblastoma (U87) was evaluated. The PC-Gd3+ were synthesized through a solvothermal process and were characterized with UV-Vis spectroscopy studies and zeta potential measurements. During the experimental phase, the biocompatibility of PC-Gd3+ was demonstrated by means of the MTT assay, since PC-Gd3+ at different concentrations did not generate cytotoxicity in cancer cells. After demonstrating the biocompatibility of the carbon dots, assays were performed to determine the cell proliferation capacity and the damage in the genetic material was calculated after exposing the cell lines incubated with the PC-Gd3+ to ionizing radiation. With these experiments, a radiosensitizing effect of PC-Gd3+ was demonstrated under specific conditions in the U87 cell line, and a tendency to decrease cell proliferation and increase damage to cellular genetic material was evidenced in the samples that were treated with PC-Gd3+. . This work is an initial work that allows establishing bases of experimentation to be able to continue studying the radiosensitizing potential of the developed nanoplatform.
