Identificación de especies del género Aedes (Diptera; Culicidae) y detección de infección por Arbovirus (CHIKV, DENV, MAYV, ZIKV) circulantes en tres municipios de Arauca, Colombia.
Thesis
The identification of the vector species and their natural infection are important data for the control of the transmission of arboviral infections. However, this information for the eastern part of Colombia is limited. Therefore, the following study aimed to identify (morphological and molecular) the species of the genus Aedes present in three municipalities (Saravena, Arauquita and Tame) in the department of Arauca, Colombia. As well as the detection of arboviral infection (Dengue, Chikungunya, Zika and Mayaro), by amplifying the genetic 3 material by RT-PCR (reverse transcriptase polymerase chain reaction). The results showed the coexistence of Ae. aegypti and Ae. albopictus in the urban area of the municipalities of Saravena and Arauquita, where the individuals were found infected by Dengue (DENV-1) and Chikungunya (CHIKV). The most frequently arbovirus was DENV-1 with an infection rate of 24.3% (27/111) for Ae. aegypti and 39.7% (23/58) for Ae. albopictus. Followed by CHIKV with an infection rate of 1.8% (2/111) for Ae. aegypti and 6.9% (4/58) for Ae. albopictus. A mixed infection of DENV-1 and CHIKV was obtained in 4.5% (5/111) in the species Ae. aegypti and Zika (ZIKV) and Mayaro (MAYV) infection were not detected. Finally, the present study proposes the individual processing of insects for the detection of arboviruses since in this way a more accurate infection rate is obtained and the mixed infections are evidenced.
