Integrating molecular profiling into glioma diagnosis: implications of the WHO-CNS5-2021 classification of adult-type diffuse gliomas in Colombian patients

Thesis author

Ana María Chinchilla
Andrés Felipe Patiño-Aldana
Daniel Felipe Silgado-Guzmán
Fernando Velandia
Hanna Valentina Tovar
José Manuel Palacio
Matteo Mineo-Pachón
Nattaly Valero
Nicolas Caballero
Omar Echeverría
Stevan Peralta
Verónica Uribe
William Mauricio Riveros Castillo

Resumen

Introduction: Gliomas are the most frequent type of primary malignant central nervous system (CNS) tumors, representing a group of heterogeneous neoplasms with variable clinical behavior that require adequate diagnostic accuracy. The identification of molecular biomarkers has recently gained significance for the diagnosis, prognosis, and treatment of CNS tumors; the application of current clinical guidelines is necessary. Our study performed a molecular characterization of gliomas in a cohort of Colombian patients using the recommendations of the 2021 World Health Organization (WHO) CNS 5 classification. Materials and methods: We analyzed 22 Colombian patients with CNS tumors. Molecular techniques including Sanger sequencing, multiplex ligation-dependent probe amplification (MLPA) and methylation-specific MLPA (MS-MLPA) were used to identify mutations in IDH1, IDH2, TERT, and EGFR, as well as 1p/19q co-deletion and MGMT promoter methylation status. Results: Our results demonstrated a 23% discordance rate between histopathologic and molecular classifications, with most of the discrepancies due to an initial histopathologic classification of glioblastomas, which were molecularly reclassified as astrocytomas. In addition, molecular profiling allowed us to identify non-canonical mutations, including IDH1 p.R132S, which has shown an impact on patient prognosis. Discussion: We highlight the importance of incorporating molecular methods to improve diagnostic accuracy and achieve personalized treatments for gliomas, as proposed by the current 2021 WHO CNS 5 tumor classification guidelines. Performing new studies with larger patient cohorts integrating clinical data is necessary to determine the behavior, epidemiology, and therapeutic outcomes of this type of tumor more comprehensively.

autores

http://vivoweb.org/ontology/core#FacultyMember
Contreras Bravo, Nora Constanza
http://vivoweb.org/ontology/core#FacultyMember
Fonseca Mendoza, Dora Janeth
http://vivoweb.org/ontology/core#FacultyMember
Ondo Méndez, Alejandro Oyono

fecha de publicación

2026-1-1

Edición

16

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VIVO

Integrating molecular profiling into glioma diagnosis: implications of the WHO-CNS5-2021 classification of adult-type diffuse gliomas in Colombian patients Artículo académico

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